GCNT3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88313

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GCNT3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RLKCDSDHLGLESRESQSQYCRNILYNFLKLPAKRSINCSGVTRGDQEAVLQAILNNLEVKKKREPFTDTHYLSLTRDCEHFKAERKFIQFPLSKEEVEFPIAYSMVIHEKIENFER

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24889196).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCNT3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GCNT3 Antibody [NBP1-88313]

Immunocytochemistry/ Immunofluorescence: GCNT3 Antibody [NBP1-88313]

Immunocytochemistry/Immunofluorescence: GCNT3 Antibody [NBP1-88313] - Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]

Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]

Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313] - Staining in human rectum and liver tissues using anti-GCNT3 antibody. Corresponding GCNT3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]

Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]

Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313] - Staining of human rectum shows high expression.
Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]

Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313]

Immunohistochemistry-Paraffin: GCNT3 Antibody [NBP1-88313] - Staining of human liver shows low expression as expected.
GCNT3 Antibody - BSA Free Western Blot: GCNT3 Antibody - BSA Free [NBP1-88313]

Western Blot: GCNT3 Antibody - BSA Free [NBP1-88313]

Analysis in human cell line A-549.
Simple Western: GCNT3 Antibody [NBP1-88313]

Simple Western: GCNT3 Antibody [NBP1-88313]

Simple Western: GCNT3 Antibody [NBP1-88313] - Simple Western lane view shows a specific band for GCNT3 in 0.5 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: GCNT3 Antibody [NBP1-88313]

Simple Western: GCNT3 Antibody [NBP1-88313]

Simple Western: GCNT3 Antibody [NBP1-88313] - Simple Western lane view shows a specific band for GCNT3 in 0.5 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: GCNT3 Antibody [NBP1-88313]

Simple Western: GCNT3 Antibody [NBP1-88313]

Simple Western: GCNT3 Antibody [NBP1-88313] - Electropherogram image(s) of corresponding Simple Western lane view. GCNT3 antibody was used at 1:20 dilution on RT-4 lysates(s).

Applications for GCNT3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 24889196). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 61-64 kDa

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GCNT3

GCNT3 catalyzes O-glycan branch synthesis of the core 2 and core 4 type in mucins and controls expression of core 2 branched oligosaccharides and I antigens on the cell surface. [provided by RefSeq]

Alternate Names

beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase 3, beta1,6GlcNAc-transferase, beta1,6-N-acetylglucosaminyltransferase-M, C2/4GnTGNTM, C24GNT, C2GnT2, C2GNTM, C2GnT-M, C2GnT-mucin type, Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase, EC 2.4.1.102, EC 2.4.1.150, glucosaminyl (N-acetyl) transferase 3, mucin type, hC2GnT-M, mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase

Gene Symbol

GCNT3

Additional GCNT3 Products

Product Documents for GCNT3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GCNT3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GCNT3 Antibody - BSA Free

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Protocols

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