GCSH Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-04472

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all GCSH Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human GCSH (NP_004474.2). MALRVVRSVRALLCTLRAVPSPAAPCPPRPWQLGVGAVRTLRTGPALLSVRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GCSH Antibody - Azide and BSA Free

GCSH Antibody - Azide and BSA Free

Western Blot: GCSH Antibody - Azide and BSA Free [GCSH] -

Western Blot: GCSH Antibody - Azide and BSA Free [GCSH] - Western blot analysis of various lysates, using GCSH Rabbit pAb at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
GCSH Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GCSH Antibody - Azide and BSA Free [GCSH] -

Immunocytochemistry/ Immunofluorescence: GCSH Antibody - Azide and BSA Free [GCSH] - Confocal immunofluorescence analysis of U2OS cells using GCSH Rabbit pAb at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.
GCSH Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GCSH Antibody - Azide and BSA Free [GCSH] -

Immunocytochemistry/ Immunofluorescence: GCSH Antibody - Azide and BSA Free [GCSH] - Immunofluorescence analysis of L929 cells using GCSH Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
GCSH Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GCSH Antibody - Azide and BSA Free [GCSH] -

Immunocytochemistry/ Immunofluorescence: GCSH Antibody - Azide and BSA Free [GCSH] - Immunofluorescence analysis of C6 cells using GCSH Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
GCSH Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: GCSH Antibody - Azide and BSA Free [GCSH] -

Immunocytochemistry/ Immunofluorescence: GCSH Antibody - Azide and BSA Free [GCSH] - Immunofluorescence analysis of U-2 OS cells using GCSH Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for GCSH Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GCSH

The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to themitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase;MIM 238300), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme; MIM238310), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899), also callednonketotic hyperglycinemia (NKH), may be due to a defect in any one of these enzymes.(supplied by OMIM)

Alternate Names

GCE, glycine cleavage system H protein, mitochondrial, glycine cleavage system protein H (aminomethyl carrier), lipoic acid-containing protein, mitochondrial glycine cleavage system H-protein, NKH

Gene Symbol

GCSH

Additional GCSH Products

Product Documents for GCSH Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GCSH Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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