Chemical Name: (+)-2'-Deoxy-2',2'-difluorocytidine hydrochloride
Biological ActivityDeoxycytidine analog that inhibits DNA synthesis. Metabolized to form gemcitabine triphosphate (dFdCTP) and gemcitabine diphosphate (dFdCDP). dFdCTD inhibits ribonucleotide reductase causing a reduction in cellular nucleotides. dFdCTP is incorporated in DNA resulting in DNA strand termination. Displays antitumor activity in vitro and in vivo.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Gemcitabine: a modulator of intracellular nucleotide and deoxynucleotide metabolism.
Heinemann et al.
Evaluation of the antitumor activity of gemcit. (2',2'-Difluoro-2'-deoxycytidine).
Hertel et al.
Cancer Res., 1990;50:4417
Preclinical characteristics of gemcit.
Plunkett et al.
Anticancer Drugs, 1995;6:7
Citations for Gemcitabine hydrochloride
The citations listed below are publications that use Tocris products. Selected citations for Gemcitabine hydrochloride include:
14 Citations: Showing 1 - 10
Targeting Multiple Effector Pathways in Pancreatic Ductal Adenocarcinoma with a G-Quadruplex-Binding Small Molecule.
Authors: Marchetti Et al.
J Med Chem 2018;61:2500
SUV420H2 is an epigenetic regulator of epithelial/mesenchymal states in pancreatic cancer.
Authors: Viotti Et al.
J Cell Biol 2018;217:763
Plk1 inhibition enhances the efficacy of gemcit. in human pancreatic cancer.
Authors: Li Et al.
Cell Cycle 2016;15:711
An automated fitting procedure and software for dose-response curves with multiphasic features.
Authors: Veroli Et al.
Mol Cancer Ther 2015;5:14701
Quantitative proteomics of the yeast Hsp70/Hsp90 interactomes during DNA damage reveal chaperone-dependent regulation of ribonucleotide reductase.
Authors: Truman Et al.
J Proteomics 2015;112:285
DNA-Dependent Protein Kinase As Molecular Target for Radiosensitization of Neuroblastoma Cells.
Authors: Dolman Et al.
PLoS One 2015;10:e0145744
Contrasting Effects of the Cytotoxic Anticancer Drug gemcit. and the EGFR Tyrosine Kinase Inhibitor gefi. on NK Cell-Mediated Cytotoxicity via Regulation of NKG2D Ligand in Non-Small-Cell Lung Cancer Cells.
Authors: Okita Et al.
PLoS One 2015;10:e0139809
Comparative Study of 17-AAG and NVP-AUY922 in Pancreatic and Colorectal Cancer Cells: Are There Common Determinants of Sensitivity?
Authors: Mayor-López Et al.
Transl Oncol 2014;7:590
Selective inhibition of pancreatic ductal adenocarcinoma cell growth by the mitotic MPS1 kinase inhibitor NMS-P715.
Authors: Slee Et al.
Mol Cancer Ther 2014;13:307
BRCA2 and RAD51 promote double-strand break formation and cell death in response to gemcit.
Authors: Jones Et al.
PLoS One 2014;13:2412
Gemcitabine diphosphate choline is a major metabolite linked to the Kennedy pathway in pancreatic cancer models in vivo.
Authors: Bapiro Et al.
Br J Cancer 2014;111:318
A simple high-content cell cycle assay reveals frequent discrepancies between cell number and ATP and MTS proliferation assays.
Authors: Chan Et al.
PLoS One 2013;8:e63583
Characterization of permeability, stability and anti-HIV-1 activity of decit. and gemcit. divalerate prodrugs.
Authors: Clouser Et al.
Antivir Chem Chemother 2013;23:223
Plk1 phosphorylation of orc2 and hbo1 contributes to gemcit. resistance in pancreatic cancer.
Authors: Song Et al.
Mol Cancer Ther 2013;12:58
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The compound was used as a control in a study where we determined the effect of our compounds on ribonucleotide reductase enzyme.
Gemcitabine was used at a 20 uM concentration for in vitro culture with SK-MEL-28 and cell viability was monitored over time with live imaging. Arrow indicates when compound was added to media.