Chemical Name: (+)-2'-Deoxy-2',2'-difluorocytidine hydrochloride
Biological ActivityDeoxycytidine analog that inhibits DNA synthesis. Metabolized to form gemcitabine triphosphate (dFdCTP) and gemcitabine diphosphate (dFdCDP). dFdCTD inhibits ribonucleotide reductase causing a reduction in cellular nucleotides. dFdCTP is incorporated in DNA resulting in DNA strand termination. Displays antitumor activity in vitro and in vivo. Inhibits replication of MERS-CoV and SARS-CoV in vitro (EC50 values are 1,2 16 and 4.957 μM, respectively).
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Tocris products are intended for laboratory research use only, unless stated otherwise.
Gemcitabine: a modulator of intracellular nucleotide and deoxynucleotide metabolism.
Heinemann et al.
Evaluation of the antitumor activity of gemcit. (2',2'-Difluoro-2'-deoxycytidine).
Hertel et al.
Cancer Res., 1990;50:4417
Preclinical characteristics of gemcit.
Plunkett et al.
Anticancer Drugs, 1995;6:7
Repurposing of clinically developed drugs for treatment of Middle East Respiratory Syndrome coronavirus infection.
Dyall et al.
Antimicrob.Agents Chemother., 2014;58:4885
Citations for Gemcitabine hydrochloride
The citations listed below are publications that use Tocris products. Selected citations for Gemcitabine hydrochloride include:
14 Citations: Showing 1 - 10
Targeting Multiple Effector Pathways in Pancreatic Ductal Adenocarcinoma with a G-Quadruplex-Binding Small Molecule.
Authors: Marchetti Et al.
J Med Chem 2018;61:2500
SUV420H2 is an epigenetic regulator of epithelial/mesenchymal states in pancreatic cancer.
Authors: Viotti Et al.
J Cell Biol 2018;217:763
Plk1 inhibition enhances the efficacy of gemcit. in human pancreatic cancer.
Authors: Li Et al.
Cell Cycle 2016;15:711
An automated fitting procedure and software for dose-response curves with multiphasic features.
Authors: Veroli Et al.
Mol Cancer Ther 2015;5:14701
Quantitative proteomics of the yeast Hsp70/Hsp90 interactomes during DNA damage reveal chaperone-dependent regulation of ribonucleotide reductase.
Authors: Truman Et al.
J Proteomics 2015;112:285
DNA-Dependent Protein Kinase As Molecular Target for Radiosensitization of Neuroblastoma Cells.
Authors: Dolman Et al.
PLoS One 2015;10:e0145744
Contrasting Effects of the Cytotoxic Anticancer Drug gemcit. and the EGFR Tyrosine Kinase Inhibitor gefi. on NK Cell-Mediated Cytotoxicity via Regulation of NKG2D Ligand in Non-Small-Cell Lung Cancer Cells.
Authors: Okita Et al.
PLoS One 2015;10:e0139809
Comparative Study of 17-AAG and NVP-AUY922 in Pancreatic and Colorectal Cancer Cells: Are There Common Determinants of Sensitivity?
Authors: Mayor-López Et al.
Transl Oncol 2014;7:590
Selective inhibition of pancreatic ductal adenocarcinoma cell growth by the mitotic MPS1 kinase inhibitor NMS-P715.
Authors: Slee Et al.
Mol Cancer Ther 2014;13:307
BRCA2 and RAD51 promote double-strand break formation and cell death in response to gemcit.
Authors: Jones Et al.
PLoS One 2014;13:2412
Gemcitabine diphosphate choline is a major metabolite linked to the Kennedy pathway in pancreatic cancer models in vivo.
Authors: Bapiro Et al.
Br J Cancer 2014;111:318
A simple high-content cell cycle assay reveals frequent discrepancies between cell number and ATP and MTS proliferation assays.
Authors: Chan Et al.
PLoS One 2013;8:e63583
Characterization of permeability, stability and anti-HIV-1 activity of decit. and gemcit. divalerate prodrugs.
Authors: Clouser Et al.
Antivir Chem Chemother 2013;23:223
Plk1 phosphorylation of orc2 and hbo1 contributes to gemcit. resistance in pancreatic cancer.
Authors: Song Et al.
Mol Cancer Ther 2013;12:58
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Reviews for Gemcitabine hydrochloride
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The compound was used as a control in a study where we determined the effect of our compounds on ribonucleotide reductase enzyme.
Gemcitabine was used at a 20 uM concentration for in vitro culture with SK-MEL-28 and cell viability was monitored over time with live imaging. Arrow indicates when compound was added to media.