GFP Alexa Fluor® 405-conjugated Antibody

    
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  • Specificity
    Detects recombinant GFPuv and eGFP in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 454505
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant GFPuv
    Ser2-Lys238
    Accession # P42212
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 405
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of eGFP in HEK293 Human Cell Line Transfected with eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with eGFP were stained with and either (A) Mouse Anti-GFP Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB42401V) or (B) Mouse IgG1 Alexa Fluor 405 Isotype Control (Catalog # IC002V). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.

  • Long Name:
    Green Fluorescent Protein
  • Alternate Names:
    eGFP; Enhanced Green Fluorescent Protein; GFP; GFPuv; green fluorescent protein (gfp); Green Fluorescent Protein

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 405-conjugated Antibody

Ctrl IC002V
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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