GFP Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB42401T-100UG
Detection  of eGFP in HEK293 Human Cell Line Transfected with eGFP by Flow Cytometry.
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GFP Alexa Fluor® 594-conjugated Antibody Summary

Specificity
Detects recombinant GFPuv and eGFP in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 454505
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant GFPuv
Ser2-Lys238
Accession # P42212
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of eGFP in HEK293 Human Cell Line Transfected with eGFP by Flow Cytometry. View Larger

Detection of eGFP in HEK293 Human Cell Line Transfected with eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with eGFP were stained with and either (A) Mouse Anti-GFP Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB42401T) or (B) Mouse IgG1 Alexa Fluor 594 Isotype Control (Catalog # IC002T). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta -barrel with a chromophore (aa 65-67) containing alpha -helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.

Long Name
Green Fluorescent Protein
Alternate Names
eGFP; Enhanced Green Fluorescent Protein; GFP; GFPuv; green fluorescent protein (gfp); Green Fluorescent Protein

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