GFP Alexa Fluor® 405-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: GFP

The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa. GFP is in the shape of a cylinder, comprising 11 strands of beta-sheet outside, a single alpha helical strand containing the chromophore running through the center, and short helical segments on the ends. GFP transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. GFP has been widely used as a tracer in various diciplines of biology research, such as fluorescence microscopy, reporter gene expression, and in vivo imaging. Laboratory mutation of GFP generated the widely used analog GFPuv, which has brighter fluorescence and more desirable expression properties.

Product Datasheets