Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 454518
View all GFP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant GFPuv
Ala2-Tyr238
Accession # P42212

Specificity

Detects GFPuv in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for GFP Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with recombinant GFPuv, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant GFPuv

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFP

The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa. GFP is in the shape of a cylinder, comprising 11 strands of beta-sheet outside, a single alpha helical strand containing the chromophore running through the center, and short helical segments on the ends. GFP transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. GFP has been widely used as a tracer in various diciplines of biology research, such as fluorescence microscopy, reporter gene expression, and in vivo imaging. Laboratory mutation of GFP generated the widely used analog GFPuv, which has brighter fluorescence and more desirable expression properties.

Long Name

Green Fluorescent Protein

Alternate Names

eGFP, GFPuv

UniProt

P42212

Additional GFP Products

Product Documents for GFP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for GFP Antibody

For research use only

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FAQs

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