Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot, ELISA, Sandwich ELISA Detection, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 208 expressed in HEK293

Format

Azide and BSA Free
View all GFP Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Aequorea victoria GFP (Accession#: AAB65663; Ser2-Lys238).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for GFP Antibody (208) - Azide and BSA Free

Western Blot: GFP Antibody (208) [NBP2-90178]

Western Blot: GFP Antibody (208) [NBP2-90178]

Western Blot: GFP Antibody (208) [NBP2-90178] - Anti-GFP rabbit monoclonal antibody at 1:500 dilution. Lane A: GFP transfected E.coli lysate (5ug). Lane B: GFP transfected 293 Cell lysate (30ug). Lane C: Non-transfected 293 Cells lysate (30ug). Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:27 kDa. Observed band size:27 kDa.
Immunoprecipitation: GFP Antibody (208) [NBP2-90178]

Immunoprecipitation: GFP Antibody (208) [NBP2-90178]

Immunoprecipitation: GFP Antibody (208) [NBP2-90178] - Anti-GFP Tag was immunoprecipitated using:Lane A: GFP transfected E.coli cell lysate (0.5mg)Lane B: GFP transfected 293 cell lysate (0.5mg) Anti-GFP Tag mouse monoclonal antibody at 1/500-1/2000 dilution and 60ug of Immunomagnetic beads Protein G.Primary antibody:Anti-GFP Tag rabbit monoclonal antibody, at 1/1000-1/5000 dilutionSecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.
GFP Antibody (208) Western Blot: GFP Antibody (208) [NBP2-90178]

Western Blot: GFP Antibody (208) [NBP2-90178]

NBP2-90178 used at 1:1000 dilution. Lane 1: Aequorea victoria GFP Protein - His. Lane 2: EGFP/Enhanced Green Fluorescent Protein - His. Lane 3: Aequorea victoria Green fluorescent protein /enhanced GFP Protein - His. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.

Applications for GFP Antibody (208) - Azide and BSA Free

Application
Recommended Usage

ELISA

1/10000-1/20000

Immunoprecipitation

1-5 ul/mg of lysate

Western Blot

1/1000-1/5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Long Name

Green Fluorescent Protein

Alternate Names

eGFP, GFPuv

Additional GFP Products

Product Documents for GFP Antibody (208) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GFP Antibody (208) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for GFP Antibody (208) - Azide and BSA Free

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    • Q: We are looking for a rabbit anti-GFP antibody as a primary antibody for immunofluorescence and WB. I've seen that you carry several anti-GFP antibodies and I would like to ask which one you would recommend. The one with Cat.No NB600-308 has been used in a lot of publications and I reckon it is a solid antibody. Is this our best choice, or is there a better alternative?
      A: NB600-308 is an excellent GFP antibody and I think it would work very well for you. It has been reviewed and published with which usually makes our customer feel much more confident, seeing as it worked in other people's hand.
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