GFP Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56401

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Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GFP Primary Antibodies »

Product Specifications

Immunogen

This GFP antibody was developed against a synthetic peptide corresponding to amino acids 102-118 (KDDGNYKTRAEVKFEGD).

Reactivity Notes

Chemical

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GFP Antibody - BSA Free

Western Blot: GFP Antibody [NB100-56401]

Western Blot: GFP Antibody [NB100-56401]

Western Blot: GFP Antibody [NB100-56401] - Analysis of extracts from 293A cells, unstransfected (lane 1) and transfected (lane 2) with GFP expression vector, using GFP antibody (NB100-56401) at 1:1000 (0.5 ug/mL).
Western Blot: GFP Antibody [NB100-56401]

Western Blot: GFP Antibody [NB100-56401]

Western Blot: GFP Antibody [NB100-56401] - Analysis of GFP in 20 ug of transfected HeLa cells (A) and untransfected HeLa cells (B) using GFP antibody at 0.5 ug/mL.

Applications for GFP Antibody - BSA Free

Application
Recommended Usage

Western Blot

0.5 - 2 ug/mL

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T.,... Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S.,... Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Long Name

Green Fluorescent Protein

Alternate Names

eGFP, GFPuv

Additional GFP Products

Product Documents for GFP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GFP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GFP Antibody - BSA Free

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FAQs for GFP Antibody - BSA Free

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  • Q: We are looking for a rabbit anti-GFP antibody as a primary antibody for immunofluorescence and WB. I've seen that you carry several anti-GFP antibodies and I would like to ask which one you would recommend. The one with Cat.No NB600-308 has been used in a lot of publications and I reckon it is a solid antibody. Is this our best choice, or is there a better alternative?

    A: NB600-308 is an excellent GFP antibody and I think it would work very well for you. It has been reviewed and published with which usually makes our customer feel much more confident, seeing as it worked in other people's hand.

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