Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all GGA3 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human GGA3 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GGA3 Antibody - Azide and BSA Free

GGA3 Antibody - Azide and BSA Free GGA3 Antibody - Azide and BSA Free

Immunoprecipitation: GGA3 Antibody - Azide and BSA Free [NBP3-49157] -

Immunoprecipitation: GGA3 Antibody - Azide and BSA Free [NBP3-49157] - Lane A: 0.5 mg SH-SY5Y Whole Cell Lysate. Blotted with 4 uL anti-GGA3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Immunoprecipitated using NBP3-49157 at 1:100 dilution and HRP-conjugated secondary antibody at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 95 kDa. Observed band size : 95 kDa

Applications for GGA3 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS (pH 7.0)

Format

Azide and BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GGA3

GGA3 encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network (TGN) and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma- adaptins. GGA3 mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Alternative splicing of this gene results in two transcript variants.

Alternate Names

ADP-ribosylation factor-binding protein GGA3, golgi associated, gamma adaptin ear containing, ARF binding protein 3, golgi-associated, gamma adaptin ear containing, ARF binding protein 3, KIAA0154Golgi-localized, gamma ear-containing, ARF-binding protein 3

Gene Symbol

GGA3

Additional GGA3 Products

Product Documents for GGA3 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GGA3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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