Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GKAP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human G Kinase Anchoring Protein 1 using the numbering given in entry NP_079487.2 (GeneID 80318).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GKAP1 Antibody - BSA Free

Western Blot: GKAP1 Antibody [NBP1-79017]

Western Blot: GKAP1 Antibody [NBP1-79017]

Western Blot: GKAP1 Antibody [NBP1-79017] - Whole cell lysate from 293T(15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 mcg) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-GKAP1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. GKAP1 was also immunoprecipitated by another rabbit anti-GKAP1 antibody which recognizes a downstream epitope.

Applications for GKAP1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10ug/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GKAP1

The GKAP1 gene encodes a G kinase-anchoring protein 1 that is thought to be involved in germ cell development as it localizes with the Golgi and recruits cGMP-dependent protein kinase I alpha to the Golgi in the mouse tests. It exists in two isoforms with isoform 1 at a length of 366 amino acids at 42 kDA and isoform 2 is 315 amino acids long at 36 kDa. The GKAP1 gene has been known to interact with other genes FXR2, HBP1, HGS, PRKG1, and UBQLN4. It has been researched regarding its role in different diseases such as leukemia, ataxia, and pancreatitis.

Alternate Names

cGMP-dependent protein kinase-anchoring protein of 42 kDa, FKSG21, FLJ25469, G kinase anchoring protein 1, G kinase-anchoring protein 1, GKAP42cGMP-dependent protein kinase anchoring protein 42kDa, protein kinase anchoring protein GKAP42

Gene Symbol

GKAP1

UniProt

NP_079487.2 (Human)

Additional GKAP1 Products

Product Documents for GKAP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GKAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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