GLI-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-23602

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Hamster (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GLI-2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human GLI-2 protein (between residues 300-400) [UniProt# P10070]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26210874).

Localization

Nuclear and cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLI-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]

Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]

Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: GLI-2 Antibody - BSA Free [NBP2-23602]

Immunohistochemistry-Paraffin: GLI-2 Antibody - BSA Free [NBP2-23602]

Immunohistochemistry-Paraffin: GLI-2 Antibody [NBP2-23602] - GLI-2 (red) and DAPI (blue) in mouse BT-474 primary tumors (breast cancer tissue). IHC-P image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]

Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]

Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - GLI-2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:50 was used. Image objective 40x.
Immunohistochemistry: GLI-2 Antibody - BSA Free [NBP2-23602]

Immunohistochemistry: GLI-2 Antibody - BSA Free [NBP2-23602]

Immunohistochemistry: GLI-2 Antibody [NBP2-23602] - Analysis of GLI-2 in mouse heart.

Applications for GLI-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:200 - 1:300

Immunohistochemistry-Frozen

reported in scientific literature (PMID 26210874)

Immunohistochemistry-Paraffin

1:200 - 1:300
Application Notes
In Immunocytochemistry/Immunofluorescence, cytoplasmic and nuclear staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP2-23602 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLI-2

Gli2, also known as THP, is a transcription factor which binds DNA through zinc finger motifs. Belonging to the C2H2-Zinc finger type subclass of the Gli family, Gli2 is a mediator of Shh (sonic hedgehog) signaling, a pathway that is aberrantly activated in many types of cancers. Specifically, it has been found that the anti-apoptotic protein BCL-2 is up regulated by Gli2, leading to carcinogenesis. The protein encoded by the Gli2 gene localizes to the cytoplasm and activates patched (Drosophila homolog) gene expression, where it has been thought to play an important role during embryogenesis. Mutations in the Gli2 gene are found in various syndromes, including Pallister-Hall Syndrome, Greig cephalopolysyndactyly syndrome, and preaxial polydactyl type IV. Recently, the interaction between the hedgehog signaling pathway and Gli2 has been implicated in medullary thyroid carcinoma (PMID: 23410206).

Long Name

GLI-Kruppel family member GLI2

Alternate Names

GLI2, THP2

Entrez Gene IDs

2736 (Human); 14633 (Mouse)

Gene Symbol

GLI2

UniProt

P10070, Q0VGT2 (Mouse)

Additional GLI-2 Products

Product Documents for GLI-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GLI-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GLI-2 Antibody - BSA Free

Customer Reviews for GLI-2 Antibody - BSA Free (1)

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Customer Images

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  • GLI-2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Mouse
    Verified Customer | Posted 08/17/2021
    Gli-2 (red) and DAPI (blue) in BT-474 primary tumors
    Citrate retrieval buffer
    GLI-2 Antibody - BSA Free NBP2-23602

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Protocols

View specific protocols for GLI-2 Antibody - BSA Free (NBP2-23602):

GLI-2 Antibody:
Immunocytochemistry Protocol

Culture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 5-10 minutes.
2. Remove the formalin and add 0.5% Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
3. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1% Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
4. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
5. To block nonspecific antibody binding incubate in 10% normal goat serum for 1 hour at room temperature.
6. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4 degrees C overnight.
7. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
8. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
10. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 ug/ml, or coverslips can be directly mounted in media containing DAPI.
11. Cells can now be viewed with a fluorescence microscope.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.

GLI-2 Antibody:
Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 degrees C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

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