GLI-3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89275

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GLI-3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLI-3 Antibody - BSA Free

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275]

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275]

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275] - Staining of human pancreas shows no nuclear positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275]

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275]

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275]

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275]

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-89275] - Staining of human spleen shows moderate to strong nuclear positivity in cells in red pulp.

Applications for GLI-3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLI-3

Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-polysyndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and craniofacial development. Two isoforms of human Gli-3 have been reported. One is the fulllength protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.

Long Name

GLI-Kruppel family member GLI3

Alternate Names

ACLS, ADD, Bph, GCPS, GLI3, PAPA1, PAPB, Pdn, PHS, PPDIV, Xt

Gene Symbol

GLI3

Additional GLI-3 Products

Product Documents for GLI-3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GLI-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail