GLP1 Antibody (03) - BSA Free

Novus Biologicals | Catalog # NBP2-21747

Novus Biologicals
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Key Product Details

Species Reactivity

Mammal

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 03

Format

BSA Free
View all GLP-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic GLP-1(9-37)

Epitope

C-terminus of GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37)

Specificity

binds GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37). Cross-reaction with C-terminally amidated GLP-1 is 0-16% in various circumstances.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Concentration for any given batch will be between 0.85 - 1.15 mg/mL.

Scientific Data Images for GLP1 Antibody (03) - BSA Free

Glucagon Antibody (03) - BSA Free [NBP2-21747] - BSA Free [NBP2-21747]
ELISA: GLP1 Antibody (03) - BSA Free [NBP2-21747]

ELISA: GLP1 Antibody (03) - BSA Free [NBP2-21747]

ELISA: Glucagon Antibody (03) - BSA Free [NBP2-21747] - The calibration curve of a sandwich assay for Non-amidated GLP-1 using NBP2-21747 as the capture antibody and NBP1-05180B as the biotinylated detection antibody.

Applications for GLP1 Antibody (03) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-21747 binds free GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37) in solution. NBP2-21747 binds free GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37) in solution. NBP2-21747 works well as a capture antibody with NBP1-05180B as a biotinylated detection antibody cross-reacting about 5% with C-terminally amidated GLP-1. NBP2-21747 can also be used as a capture antibody for detection of GLP-1 derivatives. (1) WB: NBP2-21747 can be used in Western blotting.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GLP-1

GLP-1(7-36) amide is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Long Name

Glucagon-like Peptide 1

Alternate Names

GLP1

Gene Symbol

GCG

Additional GLP-1 Products

Product Documents for GLP1 Antibody (03) - BSA Free

Certificate of Analysis

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Product Specific Notices for GLP1 Antibody (03) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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