GLP1 Antibody (10) - BSA Free

Novus Biologicals | Catalog # NBP1-05180

Novus Biologicals
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Key Product Details

Species Reactivity

Mammal

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 10

Format

BSA Free
View all GLP-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic GLP-1(7-17)

Epitope

Free N-terminus of GLP-1(7-37) and GLP-1(7-36) amide.

Reactivity Notes

The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Specificity

Binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(9-36)amide, glucagon and human GIP. Cross-reacts 0.25% with GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4. Cross-reacts with Liraglutide.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for GLP1 Antibody (10) - BSA Free

GLP1 Antibody (10) - BSA Free [NBP1-05180] - BSA Free [NBP1-05180]
ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180]

ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180]

ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - Calibration curve using NBP2-23506 as the capture antibody and NBP2-23557 as the biotinylated detection antibody. Image using the Biotin form of this antibody.
ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180]

ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180]

ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - By using the above monoclonal antibodies, it is possible to measure intact exendin-4 by a sandwich ELISA which is not subject to interference from GLP-1 or other glucagon-related peptides that may be present. NBP1-05180 is used as capture antibody in combination with a biotinylated detection antibody from the ABS 012 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated ABS 012-35 as the detection antibody.

Applications for GLP1 Antibody (10) - BSA Free

Application
Recommended Usage

ELISA

1:20000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-05180 binds free GLP-1(7-37) and free GLP-1(7-36)amide in solution. NBP1-05180 reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well. NBP1-05180 binds free exendin-4 in solution.. When measuring "active" GLP-1 NBP1-05180B works as a detection antibody in combination with NBP1-97413. NBP1-05180B can be used as a detection antibody in combination with NBP2-21747 as a capture antibody in order to measure non-amidated GLP-1 forms and cross-reacting about 5% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GLP-1

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of of GLP-1 is fully conserved in all mammalian species examined so far.

Long Name

Glucagon-like Peptide 1

Alternate Names

GLP1

Gene Symbol

GCG

Additional GLP-1 Products

Product Documents for GLP1 Antibody (10) - BSA Free

Certificate of Analysis

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Product Specific Notices for GLP1 Antibody (10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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