GLP1 Antibody (10) - BSA Free

Novus Biologicals | Catalog # NBP1-05180

Novus Biologicals
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Key Product Details

Species Reactivity

Mammal

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 10

Format

BSA Free
View all GLP-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic GLP-1(7-17)

Epitope

Free N-terminus of GLP-1(7-37) and GLP-1(7-36) amide.

Reactivity Notes

The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Specificity

Binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(9-36)amide, glucagon and human GIP. Cross-reacts 0.25% with GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4. Cross-reacts with Liraglutide.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Concentration for any given batch will be between 0.85 - 1.15 mg/mL.

Scientific Data Images for GLP1 Antibody (10) - BSA Free

GLP1 Antibody (10) - BSA Free [NBP1-05180] - BSA Free [NBP1-05180]
ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180]

ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180]

ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - Calibration curve using NBP2-23506 as the capture antibody and NBP2-23557 as the biotinylated detection antibody. Image using the Biotin form of this antibody.
ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180]

ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180]

ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - By using the above monoclonal antibodies, it is possible to measure intact exendin-4 by a sandwich ELISA which is not subject to interference from GLP-1 or other glucagon-related peptides that may be present. NBP1-05180 is used as capture antibody in combination with a biotinylated detection antibody from the ABS 012 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated ABS 012-35 as the detection antibody.

Applications for GLP1 Antibody (10) - BSA Free

Application
Recommended Usage

ELISA

1:20000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-05180 binds free GLP-1(7-37) and free GLP-1(7-36)amide in solution. NBP1-05180 reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well. NBP1-05180 binds free exendin-4 in solution.. When measuring "active" GLP-1 NBP1-05180B works as a detection antibody in combination with NBP1-97413. NBP1-05180B can be used as a detection antibody in combination with NBP2-21747 as a capture antibody in order to measure non-amidated GLP-1 forms and cross-reacting about 5% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GLP-1

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of of GLP-1 is fully conserved in all mammalian species examined so far.

Long Name

Glucagon-like Peptide 1

Alternate Names

GLP1

Gene Symbol

GCG

Additional GLP-1 Products

Product Documents for GLP1 Antibody (10) - BSA Free

Certificate of Analysis

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Product Specific Notices for GLP1 Antibody (10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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