Glucoamylase Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-78076

Novus Biologicals
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Key Product Details

Species Reactivity

Fungi

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Glucoamylase Primary Antibodies »

Product Specifications

Immunogen

Glucoamylase [Rhizopus] (Uniprot: P07683)

Reactivity Notes

Reactive with Glucoamylase from Rhizopus.
Cross reactivity against Glucoamylase from other sources is unknown

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Glucoamylase Antibody - BSA Free

Western Blot: Glucoamylase Antibody [NBP1-78076]

Western Blot: Glucoamylase Antibody [NBP1-78076]

Western Blot: Glucoamylase Antibody [NBP1-78076] - Goat anti Glucoamylase antibody was used to detect purified glucoamylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody at 4C. Detection shown was using Dylight 488 conjugated Donkey anti goat. Images were collected using the Doc System
Glucoamylase Antibody

Glucoamylase Antibody

Western Blot of Glucoamylase Antibody. Lane 1: Molecular Weight Marker. Lane 2: detection of Anti-Glucoamylase Antibody.

Applications for Glucoamylase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:25000

Western Blot

1:500-1:3000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of Glucoamylase [Rhizopus] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glucoamylase

UniProt

P07683 (Fungi)

Additional Glucoamylase Products

Product Documents for Glucoamylase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Glucoamylase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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