GluR5/GRIK1 Antibody

Novus Biologicals | Catalog # NB100-68165

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GluR5/GRIK1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence QCKQTHPTNSTS corresponding to internal region (near C-Terminus) according to NP_783300.1.

Epitope

QCKQTHPTNSTS

Specificity

This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GluR5/GRIK1 Antibody

Western Blot: GluR5/GRIK1 Antibody [NB100-68165]

Western Blot: GluR5/GRIK1 Antibody [NB100-68165]

Western Blot: GluR5/GRIK1 Antibody [NB100-68165] - (1ug/ml) staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for GluR5/GRIK1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 150 kDa band observed in human brain is reported in scientific literature (PMID: 15483117). An additional very strong band of unknown identity was also consistently observed at 60 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GluR5/GRIK1

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene.

Long Name

Glutamate Receptor 5

Alternate Names

EAA3, EEA3, GluK1, GRIK1

Entrez Gene IDs

2897 (Human); 14805 (Mouse); 29559 (Rat)

Gene Symbol

GRIK1

UniProt

NP_000821.1 (Human)

Additional GluR5/GRIK1 Products

Product Documents for GluR5/GRIK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GluR5/GRIK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GluR5/GRIK1 Antibody

Customer Reviews for GluR5/GRIK1 Antibody

There are currently no reviews for this product. Be the first to review GluR5/GRIK1 Antibody and earn rewards!

Have you used GluR5/GRIK1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies