Glutamine Synthetase Antibody (MSVA-750M)
Novus Biologicals | Catalog # NBP3-57260
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2c Kappa Clone # MSVA-750M
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Product Specifications
Immunogen
Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary) Swissprot P15104
Localization
Cytoplasm. Mitochondrion
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2c Kappa
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.
The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.
Positive Control
Postive control:Liver: A strong GS staining should be seen of centrilobular hepatocytes while staining is weak to moderate in Kupffer cells..
Negative Control
Negitve control:Liver: Periportal hepatocytes must be GS negative in the normal liver.
Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Glutamine Synthetase Antibody (MSVA-750M)
Immunohistochemistry-Paraffin:Glutamine Synthetase Antibody (MSVA-750M)NBP3-57260
Glutamine Synthetase staining is strong in centrilobular hepatocytes weak to moderate in Kupffer cells but absent in periportal hepatocytes
Immunohistochemistry-Paraffin:Glutamine Synthetase Antibody (MSVA-750M)NBP3-57260
Hepatocellular carcinoma with excessive Glutamine Synthetase positivity of all tumor cells
Immunohistochemistry-Paraffin:Glutamine Synthetase Antibody (MSVA-750M)NBP3-57260
In the skin a nuclear and cytoplasmic Glutamine Synthetase staining can be seen in the lower third and in the top layers of the squamous epithelium
Glutamine Synthetase Cancer Tissue Gallery
Glutamine Synthetase Cancer Tissue Gallery
Glutamine Synthetase Cancer Tissue Gallery
Glutamine Synthetase Cancer Tissue Gallery
Glutamine Synthetase Normal Tissue Gallery
Glutamine Synthetase Normal Tissue Gallery
Glutamine Synthetase Normal Tissue Gallery
Glutamine Synthetase Normal Tissue Gallery
Glutamine Synthetase Normal Tissue Gallery
Glutamine Synthetase Normal Tissue Gallery
Applications for Glutamine Synthetase Antibody (MSVA-750M)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Preservative
<0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Glutamine Synthetase
Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.
Alternate Names
cell proliferation-inducing protein 59, EC 4.1.1.15, EC 6.3.1.2, GLNSPIG59, Glutamate decarboxylase, glutamate-ammonia ligase, Glutamate--ammonia ligase, glutamate-ammonia ligase (glutamine synthase), glutamine synthase, glutamine synthetase, GS, PIG43, proliferation-inducing protein 43
Gene Symbol
GLUL
Additional Glutamine Synthetase Products
Product Documents for Glutamine Synthetase Antibody (MSVA-750M)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Glutamine Synthetase Antibody (MSVA-750M)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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