Glycerol Kinase Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-78018

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Key Product Details

Species Reactivity

Bacteria

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Glycerol Kinase Primary Antibodies »

Product Specifications

Immunogen

Glycerol Kinase [Cellulomonas species] (Uniprot: D0VZG4)

Reactivity Notes

Cross reactivity against Glycerol Kinase from other sources is unknown.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glycerol Kinase [Cellulomonas species]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Glycerol Kinase Antibody - BSA Free

Western Blot: Glycerol Kinase Antibody [NBP1-78018]

Western Blot: Glycerol Kinase Antibody [NBP1-78018]

Western Blot: Glycerol Kinase Antibody [NBP1-78018] - Glycerol Kinase antibody was used to detect purified Glycerol Kinase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (block 4C). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/blocking buffer 1 hr RT). Images were collected using the VersaDoc System.
Western Blot: Glycerol Kinase Antibody [NBP1-78018]

Western Blot: Glycerol Kinase Antibody [NBP1-78018]

Western Blot: Glycerol Kinase Antibody [NBP1-78018] - Analysis using Biotin conjugate of NBP1-78018. Lane 1: Glycerol Kinase. Load: 50 ng per lane. Primary antibody: Glycerol Kinase Antibody Biotin Conjugated at 1:1000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Glycerol Kinase.
Western Blot: Glycerol Kinase Antibody [NBP1-78018]

Western Blot: Glycerol Kinase Antibody [NBP1-78018]

Western Blot: Glycerol Kinase Antibody [NBP1-78018] - Both the antiserum and IgG fractions of Glycerol Kinase antibody) are shown to detect the 128,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:4,000 dilution of the primary antibody is used followed by detection using HRP Rabbit-a-Goat IgG [H&L] diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.

Applications for Glycerol Kinase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:4000-1:20000

Western Blot

1:500-1:2000
Application Notes
This product has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Glycerol Kinase [Cellulomonas species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:18,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ml deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glycerol Kinase

Glycerol kinase catalyzes the formation of glycerol 3 phosphate from ATP and glycerol. Dihydroxyacetone and L glyceraldehyde can also act as acceptors; UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway. NB - Glycerol kinase obtained from Cellulomonas spp has an observed molecular weight of 128kDa.

Alternate Names

GK, GK1, GKD, glpK, Glycerokinase

Gene Symbol

GK

UniProt

D0VZG4 (Bacteria)

Additional Glycerol Kinase Products

Product Documents for Glycerol Kinase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Glycerol Kinase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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