GLYCTK Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83292

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IRAAMERAGKQEMLLKPHSRVQVFEGAEDNLPDRDALRAALAIQQLAEGLTADDLLLVLISGGGSALLPAPIPPVTLEEKQTLTRLLAARGATIQELNTIRKALSQLKGGGLAQAAYPAQVVSLILSDVVG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLYCTK Antibody - BSA Free

Western Blot: GLYCTK Antibody [NBP1-83292]

Western Blot: GLYCTK Antibody [NBP1-83292]

Western Blot: GLYCTK Antibody [NBP1-83292] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292]

Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292]

Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.
Western Blot: GLYCTK Antibody [NBP1-83292]

Western Blot: GLYCTK Antibody [NBP1-83292]

Western Blot: GLYCTK Antibody [NBP1-83292] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292]

Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292]

Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292]

Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292]

Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292]

Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292]

Immunohistochemistry-Paraffin: GLYCTK Antibody [NBP1-83292] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Applications for GLYCTK Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLYCTK

GLYCTK, also known as Glycerate kinase, has 7 isoforms, a 523 amino acid isoform 1 that is 55 kDa, a 234 amino acid isoform 2 that is 25 k Da, a 457 amino acid isoform 3 that is 48 kDa, a 367 amino acid isoform 4 that is 39 kDa, a 240 amino acid isoform 5 that is 25 kDa, a 205 amino acid isoform 6 that is 22 kDa, and a 187 amino acid isoform 7 that is 20 kDa; acts as a catalyzer of the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. This protein is being studied for its involvement in microcephaly and seizures. GLYCTK has been linked to glycine, serine and threonine metabolism, glycerolipid metabolism, and glyoxylate and dicarboxylate metabolism pathways where interacts with TRIP13, ALDH1B1, ALDH2, ALDH7A1, and GRHPR proteins.

Alternate Names

EC 2.7.1.31, glycerate kinase, GLYCTK1, HBeAg-binding protein 2, HBeAg-binding protein 4, HBeAgBP4A, HBEBP2, HBEBP4HBeAg binding protein 4

Gene Symbol

GLYCTK

Additional GLYCTK Products

Product Documents for GLYCTK Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for GLYCTK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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