GNB5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-06495

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GNB5 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human GNB5 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for GNB5 Antibody - BSA Free

Western Blot: GNB5 Antibody [NBP3-06495]

Western Blot: GNB5 Antibody [NBP3-06495]

Western Blot: GNB5 Antibody [NBP3-06495] - Lane A: Jurkat Whole Cell LysateLane B: RT-4 Whole Cell LysateLane C: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDa(We are unsure as to the identity of these extra bands.)
Immunoprecipitation: GNB5 Antibody [NBP3-06495]

Immunoprecipitation: GNB5 Antibody [NBP3-06495]

Immunoprecipitation: GNB5 Antibody [NBP3-06495] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-GNB5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-GNB5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size :42 kDa

Applications for GNB5 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GNB5

FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma. Component of the RGS9-1-Gbeta5 complex composed of RGS9 (isoform RGS9-1), Gbeta5 (GNB5) and RGS9BP. TISSUE SPECIFICITY: Expressed in multiple tissues.

Alternate Names

FLJ37457, FLJ43714, G protein, beta subunit 5L, G protein, beta-5 subunit, GB5, Gbeta5, guanine nucleotide binding protein (G protein), beta 5, guanine nucleotide-binding protein subunit beta-5, guanine nucleotide-binding protein, beta subunit 5L, Transducin beta chain 5

Gene Symbol

GNB5

Additional GNB5 Products

Product Documents for GNB5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GNB5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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