GOLGA3 Antibody
Novus Biologicals | Catalog # NB100-2442
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-HSQSRASKEGPGE corresponding to C-Terminus according to NP_005886.2.
Localization
Cytoplasmic and Golgi apparatus
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for GOLGA3 Antibody
Western Blot: GOLGA3 Antibody [NB100-2442]
Western Blot: GOLGA3 Antibody [NB100-2442] - Staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. (0.1ug/ml)Applications for GOLGA3 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:16000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 170 kDa band observed in human HeLa lysates (calculated MW of 167 kDa band according to NP_005886.2). An additional band of unknown identity was also consistently observed at approx. 25 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GOLGA3
Alternate Names
GCP170Golgi peripheral membrane protein, golgi autoantigen, golgin subfamily a, 3, Golgi complex-associated protein of 170 kD, Golgi complex-associated protein of 170 kDa, Golgi membrane associated protein, golgin A3, Golgin subfamily A member 3, golgin-160, golgin-165, male enhanced antigen-2, MEA-2, SY2/SY10 protein
Entrez Gene IDs
2802 (Human)
Gene Symbol
GOLGA3
UniProt
Additional GOLGA3 Products
Product Documents for GOLGA3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GOLGA3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for GOLGA3 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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