GOT2 Antibody

Novus Biologicals | Catalog # NBP2-16708

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Bovine (90%), Canine (92%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GOT2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (81%), Porcine (89%), Chicken (82%).

Localization

Mitochondrion matrix; Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GOT2 Antibody

Immunocytochemistry/ Immunofluorescence: GOT2 Antibody [NBP2-16708]

Immunocytochemistry/ Immunofluorescence: GOT2 Antibody [NBP2-16708]

Immunocytochemistry/Immunofluorescence: GOT2 Antibody [NBP2-16708] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - Mouse brain. GOT2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: GOT2 Antibody [NBP2-16708]

Western Blot: GOT2 Antibody [NBP2-16708]

Western Blot: GOT2 Antibody [NBP2-16708] - 50 ug rat lung lysate/extract 10 % SDS-PAGE GOT2 antibody dilution: 1:1000.
Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - Hepatoma tissue. GOT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - RT2 xenograft, using GOT2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Knockout Validated: GOT2 Antibody [NBP2-16708]

Western Blot: GOT2 Antibody [NBP2-16708]

Western Blot: GOT2 Antibody [NBP2-16708] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GOT2 Antibody

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] -

GOT2 antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GOT2 antibody (NBP2-16708) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GOT2 Antibody

Western Blot: GOT2 Antibody [NBP2-16708] -

GOT2 antibody detects GOT2 protein by western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
10% SDS-PAGE
GOT2 antibody (NBP2-16708) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GOT2 Antibody

Western Blot: GOT2 Antibody [NBP2-16708] -

Sample (30 ug of whole cell lysate)
A: HepG2
10% SDS PAGE
NBP2-16708 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for GOT2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GOT2

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Alternate Names

aspartate aminotransferase, mitochondrial, EC 2.6.1, EC 2.6.1.1, FABP-1, FABPpm, Fatty acid-binding protein, FLJ40994, Glutamate oxaloacetate transaminase 2, glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2), KAT4, KATIV, kynurenine aminotransferase IV, mAspAT, mitAAT, Plasma membrane-associated fatty acid-binding protein, Transaminase A

Gene Symbol

GOT2

UniProt

P00505 (Human)

Additional GOT2 Products

Product Documents for GOT2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GOT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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