GPR3 Antibody

Novus Biologicals | Catalog # NBP1-00196

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GPR3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LQRHLLPASH corresponding to internal region according to NP_005272.1.

Specificity

This antibody is not expected to cross-react with GPR6 and GPR12.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GPR3 Antibody

Western Blot: GPR3 Antibody [NBP1-00196]

Western Blot: GPR3 Antibody [NBP1-00196]

Western Blot: GPR3 Antibody [NBP1-00196] - (0.3ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for GPR3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in human brain (hippocampus and frontal cortex) lysates (calculated MW of 35.0 kDa band according to NP_005272.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GPR3

GPR3, previously called ACCA (adenylate cyclase constitutive activator), is 7-transmembrane receptor that has been shown to promote cAMP accumulation. In oocytes, cAMP generated by GPR3 regulates maintenance of meiotic arrest. GPR3 is also expressed in the brain, where it has been implicated in cAMP-dependent emotional responses and modulation of amyloid-beta peptide generation.

Long Name

G Protein-coupled Receptor 3

Alternate Names

ACCA, G protein-coupled receptor 3

Entrez Gene IDs

2827 (Human); 14748 (Mouse); 266769 (Rat)

Gene Symbol

GPR3

UniProt

NP_005272.1 (Human)

Additional GPR3 Products

Product Documents for GPR3 Antibody

Certificate of Analysis

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Product Specific Notices for GPR3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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