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Key Product Details
Species Reactivity
Validated:
Human, Porcine, Canine, Monkey, Primate
Predicted:
Bat (95%), Bovine (95%), Rabbit (95%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR4.
Epitope
3rd cytoplasmic domain
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Gibbon (100%), Mouse (85%), Rat (85%).
Specificity
Human GPR4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Product can be stored undiluted at 4C for up to 1 month.
Scientific Data Images for GPR4 Antibody - BSA Free
Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]
Immunohistochemistry-Paraffin: GPR4 Antibody [NLS144] - Anti-GPR4 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.![Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]](https://resources.rndsystems.com/images/products/GPR4-Antibody-Immunohistochemistry-Paraffin-NLS144-img0004.jpg "Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]")
Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]
Immunohistochemistry-Paraffin: GPR4 Antibody [NLS144] - Analysis of anti-GPR4 antibody with human lung, respiratory epithelium.![Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]](https://resources.rndsystems.com/images/products/GPR4-Antibody-Immunohistochemistry-Paraffin-NLS144-img0005.jpg "Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]")
Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]
Immunohistochemistry-Paraffin: GPR4 Antibody [NLS144] - Analysis of anti-GPR4 antibody with human kidney, renal tubules.![Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]](https://resources.rndsystems.com/images/products/GPR4-Antibody-Immunohistochemistry-Paraffin-NLS144-img0006.jpg "Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]")
Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]
Immunohistochemistry-Paraffin: GPR4 Antibody [NLS144] - Analysis of anti-GPR4 antibody with human kidney, renal tubules at 15 ug/ml.![Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]](https://resources.rndsystems.com/images/products/GPR4-Antibody-Immunohistochemistry-Paraffin-NLS144-img0007.jpg "Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]")
Immunohistochemistry-Paraffin: GPR4 Antibody - BSA Free [NLS144]
Immunohistochemistry-Paraffin: GPR4 Antibody [NLS144] - Analysis of anti-GPR4 antibody with human lung, adenocarcinoma.Applications for GPR4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
15 ug/ml
Application Notes
.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Background: GPR4
Long Name
G Protein-coupled Receptor 4
Alternate Names
GPR19
Entrez Gene IDs
2828 (Human)
Gene Symbol
GPR4
Additional GPR4 Products
Product Documents for GPR4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GPR4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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