GPR81 Antibody
Novus Biologicals | Catalog # NBP1-51956
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Canine (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Peptide ELISA
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-QQLARQARMKKATR corresponding to internal region according to NP_115943.1.
Epitope
C-QQLARQARMKKATR
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID:30926749).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for GPR81 Antibody
Western Blot: GPR81 Antibody [NBP1-51956]
Western Blot: GPR81 Antibody [NBP1-51956] - Analysis of GPR81 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-51956 at 0.5g/ml. Primary incubation was 1 hour. Detected by chemiluminescence.Applications for GPR81 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:8000
Western Blot
0.3 - 1 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in human liver lysates (calculated MW of 39.3 kDa band according to NP_115943.1). An additional band of unknown identity was also consistently observed at 55 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GPR81
Long Name
G Protein-coupled Receptor 81
Alternate Names
FKSG80, GPR104, HCA1, HCAR1, LACR1, TA-GPCR
Entrez Gene IDs
27198 (Human)
Gene Symbol
HCAR1
UniProt
Additional GPR81 Products
Product Documents for GPR81 Antibody
Certificate of Analysis
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Product Specific Notices for GPR81 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for GPR81 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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