GR/NR3C1 Antibody (6E6)

Name: GR/NR3C1 Antibody (6E6)
Brand: Novus Biologicals
SKU: NBP2-22174
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-22174

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 6E6

View all GR/NR3C1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human GR/NR3C1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GR/NR3C1 Antibody (6E6)

Western Blot: GR/NR3C1 Antibody (6E6)BSA Free [NBP2-22174]

Western Blot: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Western blot analysis using Glucocorticoid Receptor mouse mAb against Hela cell lysate.

Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (6E6) - BSA Free [NBP2-22174]

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Immunofluorescence analysis of PC-2 cells using Glucocorticoid Receptor mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (6E6) - BSA Free [NBP2-22174]

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Glucocorticoid Receptor mouse mAb with DAB staining.

Flow Cytometry: GR/NR3C1 Antibody (6E6) - BSA Free [NBP2-22174]

Flow Cytometry: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Flow cytometric analysis of K562 cells using Glucocorticoid Receptor mouse mAb (green) and negative control (purple).

ELISA: GR/NR3C1 Antibody (6E6) - BSA Free [NBP2-22174]

ELISA: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

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Applications for GR/NR3C1 Antibody (6E6)

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GR/NR3C1

The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Tissue specificity: Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.

Long Name

Glucocorticoid Receptor

Alternate Names

NR3C1

Gene Symbol

NR3C1

Additional GR/NR3C1 Products

Product Documents for GR/NR3C1 Antibody (6E6)

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GR/NR3C1 Antibody (6E6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Nuclear Hormone Receptors and Regulators

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