GR/NR3C1 Antibody (BuGR2) [PE]

Novus Biologicals | Catalog # NB300-731PE

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep, Yeast

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays

Cited:

Immunocytochemistry/ Immunofluorescence

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # BuGR2
View all GR/NR3C1 Primary Antibodies »

Product Specifications

Immunogen

Partially purified rat GR.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic in the absence of ligand; nuclear after ligand-binding.

Specificity

Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for GR/NR3C1 Antibody (BuGR2) [PE]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [PE] [NB300-731PE] - An intracellular stain was performed on Jurkat cells with GR/NR3C1 (BuGR2) antibody NB300-731PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.

Applications for GR/NR3C1 Antibody (BuGR2) [PE]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Gel Super Shift Assays

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GR/NR3C1

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Long Name

Glucocorticoid Receptor

Alternate Names

NR3C1

Entrez Gene IDs

2908 (Human); 14815 (Mouse); 24413 (Rat)

Gene Symbol

NR3C1

Additional GR/NR3C1 Products

Product Documents for GR/NR3C1 Antibody (BuGR2) [PE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GR/NR3C1 Antibody (BuGR2) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GR/NR3C1 Antibody (BuGR2) [PE]

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GR/NR3C1 Antibody (BuGR2) [PE]

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  • Q: I'm wondering if you can tell me more about your post-conjugation Q/C process. What kind of Q/C tests do you run, and how do you determine whether the conjugation is good or bad (or said another way, passes or fails)?

    A: We QC test every newly produced lot of antibody (unconjugated). Once the unconjugated product meets our testing standards, we release that antibody for sale. When it comes to the conjugations, we are not able to individually test every single conjugation in each application, as these are made-to-order (we only conjugate what is needed to fulfill customer orders). For PE specifically, we perform a 3:1 ratio of dye to protein, purify via HLPC, calculate the F/P ratio, and concentrate/calculate end concentration. As long as the conjugated antibody meets our F/P ratio standards and concentration standards, we release it for shipment.

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail