GR/NR3C1 Antibody (BuGR2) - BSA Free

Novus Biologicals | Catalog # NB300-731

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep, Yeast

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # BuGR2

Format

BSA Free
View all GR/NR3C1 Primary Antibodies »

Product Specifications

Immunogen

Partially purified rat GR.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic in the absence of ligand; nuclear after ligand-binding.

Specificity

Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for GR/NR3C1 Antibody (BuGR2) - BSA Free

Western Blot: GR/NR3C1 Antibody (BuGR2)BSA Free [NB300-731]

Western Blot: GR/NR3C1 Antibody (BuGR2)BSA Free [NB300-731]

Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in A549 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in HeLa Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of GR in Jurkat cells compared to an isotype control (blue).
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in NIH/3T3 cells compared to an isotype control (blue).
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in HeLa cells compared to an isotype control (blue).
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) - BSA Free [NB300-731]

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - An intracellular stain was performed on Jurkat cells with GR/NR3C1 (BuGR2) antibody NB300-731PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.

Applications for GR/NR3C1 Antibody (BuGR2) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Flow Cytometry

1:10 - 1:1000

Gel Super Shift Assays

1:10 - 1:100

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

5 ug/mL

Immunoprecipitation

1:10 - 1:500

Western Blot

5 ug/mL
Application Notes
Blocking, ChIP, and ELISA usages were reported in scientific literature. Use in ICC/IF was reported in scientific literature (PMID: 30402116)

Reviewed Applications

Read 3 reviews rated 3 using NB300-731 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with 0.1 ml sterilized water to desired concentration.

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GR/NR3C1

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Long Name

Glucocorticoid Receptor

Alternate Names

NR3C1

Entrez Gene IDs

2908 (Human); 14815 (Mouse); 24413 (Rat)

Gene Symbol

NR3C1

Additional GR/NR3C1 Products

Product Documents for GR/NR3C1 Antibody (BuGR2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GR/NR3C1 Antibody (BuGR2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GR/NR3C1 Antibody (BuGR2) - BSA Free

Customer Reviews for GR/NR3C1 Antibody (BuGR2) - BSA Free (3)

3 out of 5
3 Customer Ratings
5 Stars
0%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
33%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • GR/NR3C1 Antibody (BuGR2)
    Name: Shavahn Loux
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Liver tissue and Liver
    Species: Equine
    Verified Customer | Posted 02/01/2020
    Tried HIER ph 6 and HIER pH 9 with concentrations as high as 1:200. Never saw any nuclear staining in horse liver; only non-specific background staining.
    GR/NR3C1 Antibody (BuGR2) - BSA Free NB300-731
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Name: Agnes Villers
    Application: Western Blot
    Sample Tested: mouse hippocampal tissue lysate
    Species: Mouse
    Verified Customer | Posted 09/03/2014
    Blot with anti-glucocorticoid receptor 2 (NB300-371), duplicated sample of mouse hippocampal protein extract
    GR/NR3C1 Antibody (BuGR2) - BSA Free NB300-731
  • Name: Agnes Villers
    Application: Western Blot
    Verified Customer | Posted 08/29/2014
    Blot with anti-glucocorticoid receptor 2 (NB300-371), duplicated sample of mouse hippocampal protein extract
    GR/NR3C1 Antibody (BuGR2) - BSA Free NB300-731

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail