Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 611 to 661 of human GTPase Regulator Associated with Focal Adhesion Kinase pp125(FAK) using the numbering given in entry NP_055886.1 (GeneID 23092).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GRAF Antibody - BSA Free
Western Blot: GRAF Antibody [NBP2-32229]
Western Blot: GRAF Antibody [NBP2-32229] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GRAF antibody NBP2-32229 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunoprecipitation: GRAF Antibody [NBP2-32229]
Immunoprecipitation: GRAF Antibody [NBP2-32229] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-GRAF antibody NBP2-32229 used for IP at 6 ug per reaction. For blotting immunoprecipitated GRAF, NBP2-32229 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for GRAF Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: GRAF
Long Name
Rho GTPase Activating Protein 26
Alternate Names
ARHGAP26, OPHN1L1
Gene Symbol
ARHGAP26
UniProt
Additional GRAF Products
Product Documents for GRAF Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GRAF Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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