Granulysin Antibody

Novus Biologicals | Catalog # NBP2-27101

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Granulysin Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 70-120 from human GNLY was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen for this GNLY antibody is 100% homologous in human, and Chimpanzee, and 81% homologous in orangutan.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Granulysin Antibody

Western Blot: Granulysin Antibody [NBP2-27101]

Western Blot: Granulysin Antibody [NBP2-27101]

Western Blot: Granulysin Antibody [NBP2-27101] - Analysis of GNLY using HEK293 cell lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of GNLY antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for Granulysin Antibody

Application
Recommended Usage

Western Blot

3-5ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Granulysin

GNLY (Granulysin) is characterized as a pro- inflammatory, cytolytic, 9kDa or 15kDa peptide found in granular endosomal compartments of Nk cells and cytotoxic T cells (1). GNLY's cytolytic activity, in part, has been attributed to induction of cathepsin B release from target cell lysosomes which leads a damaging barrage of mitochondrial targets and a subsequent rapid cell lysis or necroptosis (2). Pro-inflammatory activity of GNLY is exhibited by induced and increased expression of inflammatory and chemoattractant cytokines such as RANTES, MCP-1 IL-1 and IL-6 on Tcells, monocytes and other cells (1, 3). Induction of CD80 and CD86 on DCs and their production of IL-6 and TNFa also illustrate its activity as a functional enhancer of the DC-Tcell synapse driving and polarizing specific Tcell responses (3). More recently, the finding that GNLY stimulation of Dendritic Cells is integrated with TLR4 and MyD88 illustrates a newly described role as an alarmin and part of the Innate Immune Response in addition to original cytolytic and adjuvant inflammatory activity (4). Antibodies to detect GNLY are valuable for assessment of its role in the varied areas of innate immunity, inflammation, chemo-attraction and cytolytic activity.

Alternate Names

GNLY, LAG2, Lymphokine, NKG5, TLA519

Gene Symbol

GNLY

UniProt

P22749

Additional Granulysin Products

Product Documents for Granulysin Antibody

Certificate of Analysis

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Product Specific Notices for Granulysin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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