Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all GRB 14 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human GRB 14 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for GRB 14 Antibody - BSA Free

Immunohistochemistry-Paraffin: GRB 14 Antibody [NBP2-97218]

Immunohistochemistry-Paraffin: GRB 14 Antibody [NBP2-97218]

Immunohistochemistry-Paraffin: GRB 14 Antibody [NBP2-97218] - Human stomach with rabbit polyclonal antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GRB 14 Antibody [NBP2-97218]

Immunohistochemistry-Paraffin: GRB 14 Antibody [NBP2-97218]

Immunohistochemistry-Paraffin: GRB 14 Antibody [NBP2-97218] - Human gallbladder with rabbit polyclonal antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GRB 14 Antibody [NBP2-97218]

Immunohistochemistry-Paraffin: GRB 14 Antibody [NBP2-97218]

Immunohistochemistry-Paraffin: GRB 14 Antibody [NBP2-97218] - Human GRB 14 in human liver with rabbit polyclonal antibody at 1:500 dilution.
GRB 14 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: GRB 14 Antibody [NBP2-97218] -

Immunocytochemistry/Immunofluorescence: GRB 14 Antibody [NBP2-97218] - Immunofluorescence staining of GRB 14 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97218 antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.

Applications for GRB 14 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:200-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRB 14

Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific phosphotyrosine-containing receptor sequences. GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP and a proline-rich N terminus. A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7. GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment, but appears to bind to the EGF receptor poorly. An additional member of the GRB7 family, designated GRB14, contains a pleckstrin homology domain in its central region and a carboxy terminal SH2 domain.

Alternate Names

GRB14 adapter protein, growth factor receptor-bound protein 14

Gene Symbol

GRB14

Additional GRB 14 Products

Product Documents for GRB 14 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GRB 14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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