GRF2 Antibody (3D10) - Azide and BSA Free

Novus Biologicals | Catalog # H00002889-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3D10

Format

Azide and BSA Free
View all GRF2 Primary Antibodies »

Product Specifications

Immunogen

RAPGEF1 (AAH41710, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSW

Specificity

RAPGEF1 - Rap guanine nucleotide exchange factor (GEF) 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GRF2 Antibody (3D10) - Azide and BSA Free

Western Blot: GRF2 Antibody (3D10) [H00002889-M01]

Western Blot: GRF2 Antibody (3D10) [H00002889-M01]

Western Blot: GRF2 Antibody (3D10) [H00002889-M01] - Analysis of RAPGEF1 expression in transfected 293T cell line by RAPGEF1 monoclonal antibody (M01), clone 3D10.Lane 1: RAPGEF1 transfected lysate(122.7 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: GRF2 Antibody (3D10) [H00002889-M01] - Detection limit for recombinant GST tagged RAPGEF1 is approximately 0.1ng/ml as a capture antibody.

Applications for GRF2 Antibody (3D10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GRF2

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.

Alternate Names

C3GGRF2guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene), CRK SH3-binding GNRP, DKFZp781P1719, Guanine nucleotide-releasing factor 2, Protein C3G, Rap guanine nucleotide exchange factor (GEF) 1, rap guanine nucleotide exchange factor 1

Entrez Gene IDs

2889 (Human)

Gene Symbol

RAPGEF1

OMIM

600303 (Human)

UniProt

AAH41710 (Human)

Additional GRF2 Products

Product Documents for GRF2 Antibody (3D10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GRF2 Antibody (3D10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GRF2 Antibody (3D10) - Azide and BSA Free

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