Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein

Name: Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein
Brand: Novus Biologicals
SKU: DL-1207-NB
Price: 289 USD
Availability: InStock

Novus Biologicals | Catalog # DL-1207-NB

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Conjugate

DyLight 594 (Excitation= 593 nm, Emission= 618 nm)

Applications

Immunocytochemistry/ Immunofluorescence, Bioactivity

View all Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Proteins and Enzymes »

Product Specifications

Description

A biologically active protein corresponding to Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4.

Source: Griffonia (Bandeiraea) simplicifolia seeds

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues.

DyLight® 594 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight® 594 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.

Excitation maximum is at 592 nm and emission maximum is at 617 nm (red).
Specific for galactose. Inhibitors: 500 mM galactose or 100 mM raffinose

Purity

Affinity purified

Predicted Molecular Mass

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This "B"-rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues.

Application Notes

For most applications, the recommended concentration range is 5 - 20 ug/mL. For immunofluorescence applications, it is recommended that sections be mounted in VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium (H-1400-NB) or VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI (H-1500-NB)..

Protein / Peptide Type

Native Protein

Formulation, Preparation, and Storage

DL-1207-NB

Formulation	10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mM Ca2+
Preservative	0.08% Sodium Azide
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C in the dark.

Background: Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for alpha-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.

DyLight(R) 594 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight(R) 594 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.

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Product Specific Notices for Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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