Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-62145

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Human, mouse and rat cell lysate, serum (100 uL)

Sensitivity

8.4ng/ml

Assay Range

1.4-4500ng/ml
View all GRP78/HSPA5 ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human, Mouse, Rat

Specificity

No cross-reactivity with other similar molecules (Grp94, PDI, Calreticulin) detected using this assay.

Precision

Intra-Assay Precision (Precision within an assay) Low Concentration %CV - 14.1% High Concentration %CV - 4.5%

Inter-Assay Precision (Precision between assays) Low Concentration %CV - 13.1% High Concentration %CV - 4.8%

Recovery for Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric)

Recovery

HeLa Cell Lysate Low - 90%
Medium - 108%
High - 109% C6 Cell Lysate Low - 101%
Medium - 93%
High - 82% 3T3 Cell Lysate Low - 98%
Medium - 97%
High - 94% Serum Low - 113%
Medium - 110%
High - 120%

Scientific Data Images for Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric)

ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145]

ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145]

ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - A typical standard curve for GRP78/HSPA5 ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145]

ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145]

ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - To assess parallelism, HeLa, 3T3, and C6 lysates and serum were serially diluted into assay buffer and run in the assay. The GRP78 concentration in each sample was assigned using the standard curve. Assigned concentrations were plotted as a function of sa
ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145]

ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145]

ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - ELISA versus Western blot analysis correlation comparison of GRP78/HSPA5 using C6 cell lysates. Lysates were serially diluted and either run in the ELISA or run on a 12% Tris-glycine gel, transferred to nitrocellulose membrane and probed for GRP78 with a
ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145]

ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145]

ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - HeLa cells were treated for 5 hours with 5 uM, 50 uM or 200 uM concentrations of cyclosporine A. Following the incubation, treated and untreated cells were lysed with Extraction Reagent #2 as described above. The lysates were then evaluated in the assay.

Kit Contents for Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric)

  • Antibody
  • Assay Buffer
  • Conjugate
  • Extraction Reagent
  • Microtiter Plate
  • Standard
  • Stop Solution 2
  • TMB Substrate
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GRP78/HSPA5

Within the ER, misfolded proteins are detected by Bip (GPR78). In the presence of misfolded proteins, GPR78 disscociates from PERK, allowing it to homodimerize and autophosphoyate. In its dimerized phosphorylated form, PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. GRP78 also binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up regulate transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

Additional GRP78/HSPA5 Products

Product Documents for Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric)

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