Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 34-5-8S
View all H-2Dd Primary Antibodies »

Product Specifications

Immunogen

BDF1 mouse splenocytes

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

A conformational epitope on the alpha1 and alpha2 chains of mouse H-2Dd class I alloantigen

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for H-2Dd Antibody (34-5-8S) [Biotin]

Flow Cytometry: H-2Dd Antibody (34-5-8S) [Biotin] [NBP1-28187] - DBA/2 mouse splenocytes were stained with Mouse Anti-Mouse H-2Dd-BIOT followed by Streptavidin-FITC.
Flow Cytometry: H-2Dd Antibody (34-5-8S) [Biotin] [NBP1-28187] - Analysis of DBA/2 splenocytes.

Applications for H-2Dd Antibody (34-5-8S) [Biotin]

Application
Recommended Usage

Flow Cytometry

1 ug/10^6 cells

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: H-2Dd

MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpaha1 and alpha2 chains when complexed with beta2-microglobulin. (2) The antibody does not react with H-2Dd alpha chains synthesized in vitro. (3) Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. (4) Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. (2) MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells. (5-9)

Alternate Names

H2Dd, MHC class I

Additional H-2Dd Products

Product Documents for H-2Dd Antibody (34-5-8S) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for H-2Dd Antibody (34-5-8S) [Biotin]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for H-2Dd Antibody (34-5-8S) [Biotin]

There are currently no reviews for this product. Be the first to review H-2Dd Antibody (34-5-8S) [Biotin] and earn rewards!

Have you used H-2Dd Antibody (34-5-8S) [Biotin]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies