Streptavidin Native Protein

Novus Biologicals | Catalog # NBP2-29370

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Key Product Details

Accession Number

P22629

Conjugate

HRP

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
View all Streptavidin Proteins and Enzymes »

Product Specifications

Description

Native Bacteria Streptavidin

Source: Chromatographically purified streptavidin (Accession #:P22629)

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Purity

Chromatography

Applications

Immunohistochemistry (1:1000 - 1:5000)
Western Blot (1:10000 - 1:40000)
ELISA (1:20000 - 1:200000)

Application Notes

This product has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. This product can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Protein / Peptide Type

Native Protein

Scientific Data Images for Streptavidin Native Protein

Western Blot: Streptavidin Native Protein [NBP2-29370]

Western Blot: Streptavidin Native Protein [NBP2-29370]

Western Blot: Streptavidin Full length Protein [NBP2-29370] - Lane 1: Human IL-7. Lane 2: none. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none.
Streptavidin Native Protein

Dot Blot: Streptavidin Native Protein [NBP2-29370] -

Dot Blot Results of Streptavidin Peroxidase Conjugate. Row A: BSA-Biotin Conjugated. Row B: BSA. Sample dilutions: 1- 100ng, 2- 33.33ng, 3- 11.11ng, 4-3.7ng, 5- 1.23ng. Streptavidin Peroxidase Conjugated at 1.0µg/mL for 30mins at RT.
Streptavidin Native Protein

Dot Blot: Streptavidin Native Protein [NBP2-29370] -

Dot Blot of Human IgG F(c) Fragment Biotin Conjugated using Streptavidin HRP. Human IgG F(c) Biotin Conjugate (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Streptavidin HRP at 1:40,000 for 30 mins at RT. Block: BlockOut buffer at RT for 30 mins. Exposure: 1 sec.

Formulation, Preparation, and Storage

NBP2-29370
Preparation Method This product was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.
Formulation Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% Gentamicin Sulfate
Concentration Lyoph
Reconstitution Reconstitute with 1.0 ml deionized water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Streptavidin

Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).

Alternate Names

SA V1; SA V2; Streptavidin V1; Streptavidin V2

UniProt

P22629 (Bacteria)

Additional Streptavidin Products

Product Documents for Streptavidin Native Protein

Certificate of Analysis

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Product Specific Notices for Streptavidin Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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