H1F0 Antibody (AE-4) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48077

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # AE-4

Format

IHC-Prediluted
View all H1F0 Primary Antibodies »

Product Specifications

Immunogen

Nuclei of human leukemia biopsy cells

Localization

Nuclear

Marker

Pan Nuclear Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for H1F0 Antibody (AE-4) - IHC-Prediluted

Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077]

Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077]

Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077] - Human Tonsil stained with H1F0 antibody (AE-4) - IHC-Prediluted
Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077]

Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077]

Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with H1F0 antibody (AE-4) - IHC-Prediluted
Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077]

Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077]

Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077] - Formalin-fixed, paraffin-embedded human Pancreas stained with H1F0 antibody (AE-4) - IHC-Prediluted
H1F0 Antibody (AE-4) - IHC-Prediluted Western Blot: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077] -

Western Blot: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077] -

Western Blot Analysis of human heart tissue lysate using H1F0 Antibody (AE-4) - IHC-Prediluted.
H1F0 Antibody (AE-4) - IHC-Prediluted Immunocytochemistry/ Immunofluorescence: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077] -

Immunocytochemistry/ Immunofluorescence: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077] -

Immunofluorescence staining of PFA-fixed HeLa cells using H1F0 Antibody (AE-4) - IHC-Prediluted followed by goat anti-mouse IgG-CF488 (green). Membrane stained by Phalloidin
H1F0 Antibody (AE-4) - IHC-Prediluted Flow Cytometry: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077] -

Flow Cytometry: H1F0 Antibody (AE-4) - IHC-Prediluted [NBP2-48077] -

Flow Cytometric Analysis of HeLa cells. H1F0 Antibody (AE-4) - IHC-Prediluted followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications for H1F0 Antibody (AE-4) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: H1F0

Plasmid: pCMV-H1F0 Full length; Host Cell: 293T

Alternate Names

H1 histone family, member 0, H1.0, H1(0), H1-0, H10, H1FVMGC5241, Histone H1', Histone H1(0), histone H1.0

Gene Symbol

H1-0

Additional H1F0 Products

Product Documents for H1F0 Antibody (AE-4) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for H1F0 Antibody (AE-4) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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