HADH Antibody

Novus Biologicals | Catalog # NB100-77343

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HADH Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YERGDASKEDID corresponding to internal region according to NP_005318.2.

Epitope

YERGDASKEDID

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HADH Antibody

Western Blot: HADH Antibody [NB100-77343]

Western Blot: HADH Antibody [NB100-77343]

Western Blot: HADH Antibody [NB100-77343] - HEK293 overexpressing HADH with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with (mock transfection in first and last lanes).
Western Blot: HADH Antibody [NB100-77343]

Western Blot: HADH Antibody [NB100-77343]

Western Blot: HADH Antibody [NB100-77343] - Analysis of HADH using NB100-77343 at 0.02ug/ml in Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for HADH Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 33 kDa band observed in human heart, muscle and kidney lysates (calculated MW of 34.3 kDa band according to NP_005318.2). In transfected HEK293 transiently expressing HADH a band of approx. 38 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HADH

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene.

Alternate Names

EC 1.1.1, EC 1.1.1.35, HADH1, HADHSChydroxyacyl-Coenzyme A dehydrogenase, HCDH, hydroxyacyl-CoA dehydrogenase, L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, MGC8392, mitochondrial, MSCHAD, SCHADHHF4

Entrez Gene IDs

3033 (Human); 15107 (Mouse)

Gene Symbol

HADH

UniProt

NP_005318.2 (Human)

Additional HADH Products

Product Documents for HADH Antibody

Certificate of Analysis

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Product Specific Notices for HADH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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