Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HCLS1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human Hematopoietic Cell-specific Lyn Substrate 1 using the numbering given in entry NP_005326.2 (GeneID 3059).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HCLS1 Antibody - BSA Free

Western Blot: HCLS1 Antibody [NBP2-04028]

Western Blot: HCLS1 Antibody [NBP2-04028]

Western Blot: HCLS1 Antibody [NBP2-04028] - Detection of human HS1 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HS1 antibody NBP2-04028 used for WB at 1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. HS1 was also immunoprecipitated by another rabbit anti-HS1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Applications for HCLS1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HCLS1

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling forboth clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression

Long Name

Hematopoietic Cell-specific Lyn Substrate 1

Alternate Names

Cortactin-like, CTTNL, LckBP1

Gene Symbol

HCLS1

Additional HCLS1 Products

Product Documents for HCLS1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HCLS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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