HDAC3 Antibody (1M1O7)

Novus Biologicals | Catalog # NBP3-15760

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1M1O7 expressed in HEK293
View all Histone Deacetylase 3/HDAC3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 329-428 of human HDAC3 (NP_003874.2). FEYFAPDFTLHPDVSTRIENQNSRQYLDQIRQTIFENLKMLNHAPSVQIHDVPADLLTYDRTDEADAEERGPEENYSRPEAPNEFYDGDHDNDKESDVEI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit HDAC3 Antibody (1M1O7) (NBP3-15760) is a recombinant monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HDAC3 Antibody (1M1O7)

Western Blot: HDAC3 Antibody (1M1O7) [NBP3-15760]

Western Blot: HDAC3 Antibody (1M1O7) [NBP3-15760]

Western Blot: HDAC3 Antibody (1M1O7) [NBP3-15760] - Western blot analysis of extracts of various cell lines, using HDAC3 antibody (NBP3-15760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
Immunoprecipitation: HDAC3 Antibody (1M1O7) [NBP3-15760]

Immunoprecipitation: HDAC3 Antibody (1M1O7) [NBP3-15760]

Immunoprecipitation: HDAC3 Antibody (1M1O7) [NBP3-15760] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug HDAC3 antibody (NBP3-15760). Western blot was performed from the immunoprecipitate using HDAC3 (NBP3-15760) at a dilition of 1:1000.

Applications for HDAC3 Antibody (1M1O7)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 3/HDAC3

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Long Name

Histone Deacetylase 3

Alternate Names

HDAC3, KDAC3, RPD3-2, SMAP45

Gene Symbol

HDAC3

Additional Histone Deacetylase 3/HDAC3 Products

Product Documents for HDAC3 Antibody (1M1O7)

Certificate of Analysis

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Product Specific Notices for HDAC3 Antibody (1M1O7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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