HDAC6 Antibody

Western Blot: HDAC6 Antibody [NBP1-45611]

Western Blot: HDAC6 Antibody [NBP1-45611] - Analysis of HDAC-6 expression with Jurkat cell lysate.

Western Blot: HDAC6 Antibody [NBP1-45611] -

Senescent intestinal cells reorganize microtubules.a Microtubule stability was evaluated with nocodazole for IECs treated with vehicle (V), sodium butyrate (NaBu), or lopinavir. Disassembly or partial disassembly was scored10. Data were normalized to vehicle controls. Average + SEM are shown for three independent experiments. For each experiment, 100–157 cells were examined for every time point and condition. b Immunocytochemistry revealed changes in the abundance and organization of alpha -tubulin acetylated on K40 and total alpha -tubulin (Tubulin). DAPI stained nuclei; scale bar is 20 um. Graphs depict pixel intensities/cells normalized to vehicle controls (V). At least 135 cells were assessed for each condition. Bars represent average + SEM. c Semiquantitative Western blotting determined the abundance of alpha -tubulin acetylated on K40 (Ac-K40-Tubulin) and total alpha -tubulin. Actin was used as a loading control. Between three and seven independent experiments were evaluated by Western blotting. d Western blotting determined the abundance of HDAC6, Rock1, and Rock2 for the conditions depicted. Graphs represent averages + SEM for 3–8 independent experiments. a, b Student’s t-test evaluated differences between control and senescent cells; *p < 0.05; **p < 0.01; ***p < 0.001. c, d The molecular masses of marker proteins in kDa are indicated at the right margin of blots. One-way ANOVA combined with Bonferroni correction identified significant differences between the vehicle control and treated cells. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36526654), licensed under a CC-BY license. Not internally tested by Novus Biologicals.