Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Canine, Primate

Predicted:

Chimpanzee (100%), Equine (100%), Opossum (100%), Orangutan (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HDAC9 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 100-150 of human HDAC-9 was used as the immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC9 Antibody - BSA Free

Western Blot: HDAC9 AntibodyBSA Free [NBP2-03993]

Western Blot: HDAC9 AntibodyBSA Free [NBP2-03993]

Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for HDAC9 Antibody - BSA Free

Application
Recommended Usage

Western Blot

0.5-2 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP2-03993 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HDAC9

HDAC-9 is a histone deacetylase with an important role in the regulation of gene expression and hematopoiesis. A nuclear protein, HDAC-9 belongs to the histone deacetylase family with several isoforms and is inhibited by Trichostatin A (TSA) and suberoylanilide hydroxamic acid. It helps in deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. MEF2-dependent transcription is repressed by HDAC-9 by recruiting HDAC-1 and/or HDAC-3. It also inhibits skeletal myogenesis and is involved in heart development. Ubiquitous but variable expression is seen in brain and other tissues like heart, pancreas and also in human bladder carcinoma cells. Lack of HDAC-9 protein is found in a family with Peters anomaly, a central corneal leukoma.

Long Name

Histone deacetylase 9

Alternate Names

HD7b, HD9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR

Gene Symbol

HDAC9

UniProt

AAI11736.1 (Human)

Additional HDAC9 Products

Product Documents for HDAC9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HDAC9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HDAC9 Antibody - BSA Free

Customer Reviews for HDAC9 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human Coronary Artery Endothelial Cell lysate
    Species: Human
    Verified Customer | Posted 07/15/2016
    HDAC9 in Human Coronary Artery Endothelial Cells
    HDAC9 Antibody - BSA Free NBP2-03993

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Protocols

View specific protocols for HDAC9 Antibody - BSA Free (NBP2-03993):

HDAC9 Antibody:
Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

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