HDGFRP3 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00050810-D01P

Novus Biologicals
Loading...

Key Product Details

Species Reactivity

Human

Applications

Validated:

Western Blot

Cited:

Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
Loading...

Product Specifications

Immunogen

HDGFRP3 (NP_057157.1, 1 a.a. - 203 a.a.) full-length human protein. MARPRPREYKAGDLVFAKMKGYPHWPARIDELPEGAVKPPANKYPIFFFGTHETAFLGPKDLFPYKEYKDKFGKSNKRKGFNEGLWEIENNPGVKFTGYQAIQQQSSSETEGEGGNTADASSEEEGDRVEEDGKGKRKNEKAGSKRKKSYTSKKSSKQSRKSPGDEDDKDCKEEENKSSSEGGDAGNDTRNTTSDLQKTSEGT

Specificity

HDGFRP3 - hepatoma-derived growth factor, related protein 3,

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDGFRP3 Antibody - Azide and BSA Free

Western Blot: HDGFRP3 Antibody [H00050810-D01P]

Western Blot: HDGFRP3 Antibody [H00050810-D01P]

Western Blot: HDGFRP3 Antibody [H00050810-D01P] - Analysis of HDGFRP3 expression in transfected 293T cell line by HDGFRP3 polyclonal antibody.Lane 1: HDGFRP3 transfected lysate(22.60 KDa).Lane 2: Non-transfected lysate.

Applications for HDGFRP3 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HDGFRP3

Enhances DNA synthesis and may play a role in cell proliferation

Alternate Names

HDGF2, HDGF-2, Hepatoma-derived growth factor 2, hepatoma-derived growth factor, related protein 3, hepatoma-derived growth factor-related protein 3, HRP-3

Entrez Gene IDs

50810 (Human)

Gene Symbol

HDGFL3

Additional HDGFRP3 Products

Product Documents for HDGFRP3 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HDGFRP3 Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for HDGFRP3 Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review HDGFRP3 Antibody - Azide and BSA Free and earn rewards!

Have you used HDGFRP3 Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for HDGFRP3 Antibody - Azide and BSA Free

Showing  1 - 1 of 1 FAQ Showing All
  • Q: A while back I ordered around 18 antibodies and have been in the process of testing them. And I realized that a couple of them under the application section on the website said: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. I was wondering if that means the antibody won't detect the endogenous epitope and will only detect the recombinant protein that's been transfected? Or if it can be used also to detect the endogenously expressed protein. So the two antibodies in question are the H00050810-D01P Hdgfrp3 antibody and the H00000440-D01P Asparagine synthetase antibody. Both show a transfected lysate as a positive control for the antibodies. Are you aware of any positive control cell lines for detecting endogenous (non-transfected) protein for either or both of these antibodies?

    A: The antibodies you described should be able to detect the endogenous protein, and we guarantee them for this purpose. We do our best to provide as much information as possible about the testing conditions of our antibodies, which is why we included the stated information. I do not know of any non-transfected cell lines that are suitable as specific positive controls for either asparagine synthetase or Hdgfrp3. K563 cells and muscle tissue express asparagine synthetase, but this could be influenced by treatment of the cells. MCF-7 cells may be a good positive control for Hdgfrp3.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies
Loading...