HE4/WFDC2 Antibody (17D2) - BSA Free
Novus Biologicals | Catalog # NBP3-26252
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Scientific Data Images for HE4/WFDC2 Antibody (17D2) - BSA Free
Western Blot: HE4/WFDC2 Antibody (17D2) [NBP3-26252] -
Western Blot: HE4/WFDC2 Antibody (17D2) [NBP3-26252] - Positive Western Blot detected in: HT29 whole cell lysate, Hela whole cell lysate, HT29 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate.All lanes: HE4/WFDC2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Flow Cytometry: HE4/WFDC2 Antibody (17D2) [NBP3-26252] -
Flow Cytometry: HE4/WFDC2 Antibody (17D2) [NBP3-26252] - Overlay Peak curve showing MCF-7 cells stained with HE4/WFDC2 Antibody (17D2) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.Applications for HE4/WFDC2 Antibody (17D2) - BSA Free
Flow Cytometry
Western Blot
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
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Background: HE4/WFDC2
Long Name
Alternate Names
Gene Symbol
Additional HE4/WFDC2 Products
Product Documents for HE4/WFDC2 Antibody (17D2) - BSA Free
Certificate of Analysis
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Product Specific Notices for HE4/WFDC2 Antibody (17D2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
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FAQs for HE4/WFDC2 Antibody (17D2) - BSA Free
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Q: I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?
A:
At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following here