HE4/WFDC2 Antibody

Novus Biologicals | Catalog # NBP1-49888

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HE4/WFDC2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence GAEKTGVCPELQADQ corresponding to internal region according to NP_006094.3.

Epitope

GAEKTGVCPELQADQ - internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HE4/WFDC2 Antibody

Western Blot: HE4/WFDC2 Antibody [NBP1-49888]

Western Blot: HE4/WFDC2 Antibody [NBP1-49888]

Western Blot: HE4/WFDC2 Antibody [NBP1-49888] - Staining of Human Testes lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.

Applications for HE4/WFDC2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:1000

Western Blot

0.01 - 0.1 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris, pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HE4/WFDC2

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Long Name

WAP Four-disulfide Core Domain protein 2

Alternate Names

EDDM4, WAP5, WFDC2

Entrez Gene IDs

10406 (Human)

Gene Symbol

WFDC2

UniProt

NP_006094.3 (Human)

Additional HE4/WFDC2 Products

Product Documents for HE4/WFDC2 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for HE4/WFDC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HE4/WFDC2 Antibody

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FAQs for HE4/WFDC2 Antibody

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  • Q: I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?

    A:

    At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following here

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