Recombinant Human HE4/WFDC2 Protein

Novus Biologicals | Catalog # NBP3-07109

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Western Blot, ELISA
View all HE4/WFDC2 Proteins and Enzymes »

Product Specifications

Description

A single polypeptide chain containing 94 amino acids corresponding to HE4/WFDC2. However it migrates with an apparent molecular mass of 169 kDa in SDS-PAGE Source: E. coli

Amino Acid Sequence: EKTGVCPELQ ADQNCTQECV SDSECADNLK CCSAGCATFC SLPNDKEGSC PQVNINFPQL GLCRDQCQVD SQCPGQMKCC RNGCGKVSCV TPNF

Purity

>95%, by SDS-PAGE

Endotoxin Level

Less than 1.0 EU/ug of HE4/WFDC2 as determined by LAL method.

Predicted Molecular Mass

10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP3-07109
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.
Preservative No Preservative
Concentration Lyoph
Reconstitution Briefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HE4/WFDC2

The HE4 gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members

Long Name

WAP Four-disulfide Core Domain protein 2

Alternate Names

EDDM4, WAP5, WFDC2

Gene Symbol

WFDC2

Additional HE4/WFDC2 Products

Product Documents for Recombinant Human HE4/WFDC2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Recombinant Human HE4/WFDC2 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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FAQs for Recombinant Human HE4/WFDC2 Protein

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    • Q: I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?
      A: At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following here
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