Helicobacter Pylori Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-33100

Novus Biologicals

Key Product Details

Species Reactivity

H. pylori

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Total sonicate of Helicobacter pylori

Reactivity Notes

Helicobacter pylori.

Localization

Cell surface and cytoplasm

Specificity

The spiral shaped bacterium Helicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

1.0 mg/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-29479).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Helicobacter Pylori Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100]

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100]

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100] - Formalin-fixed. paraffin-embedded human stomach stained with helicobacter pylori rabbit polyclonal antibody.
Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100]

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100]

Immunohistochemistry-Paraffin: Helicobacter Pylori Antibody - Azide and BSA Free [NBP2-33100] - Formalin-fixed, paraffin-embedded human stomach stained with Helicobacter pylori antibody (1:200), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues requires boiling tissue sections in citrate buffer, pH 6, or 1 mM EDTA, pH 8.0

Applications for Helicobacter Pylori Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Helicobacter Pylori

This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Alternate Names

H. pylori

Additional Helicobacter Pylori Products

Product Documents for Helicobacter Pylori Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Helicobacter Pylori Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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