Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Helios Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human IKAROS Family Zinc Finger 2 (Helios) using the numbering given in entry NP_057344.2 (GeneID 22807).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Helios Antibody - BSA Free (NBP2-04050) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Helios Antibody - BSA Free

Western Blot: Helios Antibody [NBP2-04050]

Western Blot: Helios Antibody [NBP2-04050]

Western Blot: Helios Antibody [NBP2-04050] - Detection of human Helios by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Helios antibody NBP2-04050 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. Helios was also immunoprecipitated by another rabbit anti-Helios antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Immunoprecipitation: Helios Antibody [NBP2-04050]

Immunoprecipitation: Helios Antibody [NBP2-04050]

Immunoprecipitation: Helios Antibody [NBP2-04050] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Helios antibody NBP1-97741 used for IP at 6ug/mg lysate. Helios was also immunoprecipitated by rabbit anti-Helios antibody NBP2-04050, which recognizes an upstream epitope. For blotting immunoprecipitated Helios, NBP2-04050 was used at 0.4ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. nds.

Applications for Helios Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Helios

HELIOS encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

Long Name

DNA-binding Protein Helios

Alternate Names

IKZF2, ZNF1A2, ZNFN1A2

Gene Symbol

IKZF2

UniProt

Q9UKS7 (Human)

Additional Helios Products

Product Documents for Helios Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Helios Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Regulatory T Cells (Tregs)

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