Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot, Sandwich ELISA, Microarray

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Hemoglobin Primary Antibodies »

Product Specifications

Immunogen

Purified human hemoglobin A1 of adult red blood cell origin

Specificity

This antibody reacts specifically with human hemoglobin A1, A2, F and S. There is no reaction with human serum proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Hemoglobin Antibody - BSA Free

Hemoglobin alpha Antibody - BSA Free

Western Blot: Hemoglobin Antibody - BSA Free [NB110-41083] -

Whole cell lysate (50 µg) from K-562, and HeLa cells prepared using NETN lysis buffer. Antibody: Hemoglobin Antibody - BSA Free (NB110-41083) used for WB at 1:1000. Secondary: HRP-conjugated Donkey anti-Goat IgG Heavy and Light Chain Cross-Adsorbed Antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for Hemoglobin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Western Blot

1:1000 - 1:30000
Application Notes
ELISA for coating plates 1:100 - 1:500.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Hemoglobin

Hemoglobin is involved in oxygen transport from the lung to the various peripheral tissues.The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult Hemoglobin. The normal adult Hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta zero thalassemia. Reduced amounts of detectable beta globin causes beta plus thalassemia.

Alternate Names

alpha one globin;alpha-1 globin;alpha-1-globin;alpha-2 globin chain;Alpha-globin;ALPHA-GLOBIN GENE;delta globin;Haemoglobin;Hb;HbA1c;hemoglobin alpha 1 globin chain;Hemoglobin alpha chain;Hemoglobin subunit alpha;hemoglobin, alpha 1

Gene Symbol

HBA1

Additional Hemoglobin Products

Product Documents for Hemoglobin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Hemoglobin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Red Blood Cells (RBCs)

Citations for Hemoglobin Antibody - BSA Free

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FAQs for Hemoglobin Antibody - BSA Free

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  • Q: Is this the same antibody as #NB110-41080, but the unconjugated format?

    A: Yes, NB110-41083 is the same antibody as NB110-41080 but unconjugated.

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